Composite

Part:BBa_K3514004:Design

Designed by: Lukas Pranter, Oona Jung, Dominik Wiesinger   Group: iGEM20_BOKU-Vienna   (2020-10-22)


Lambda Red System


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Recombination Proteins Exo, beta, gam, each one flanked by T7Lac promoter from the registry aswell as the B1001 terminator 

For further information click on the desired Parts

Source

Exo: NCBI Reference Sequence: NP_040616.1

Beta: NCBI Reference Sequence: NP_040617.1

Gam: NCBI Reference Sequence: NP_040618.1

References

Christopher R. T. Hillyar, Genetic recombination in bacteriophage lambda, Bioscience Horizons: The International Journal of Student Research, Volume 5, 2012, hzs001, https://doi.org/10.1093/biohorizons/hzs001